Transgenic lettuce seedlings carrying hepatitis B virus antigen HBsAg.

نویسندگان

  • Jackson Marcondes
  • Ekkehard Hansen
چکیده

The obtainment of transgenic edible plants carrying recombinant antigens is a desired issue in search for economic alternatives viewing vaccine production. Here we report a strategy for genetic transformation of lettuce plants (Lactuca sativa L.) using the surface antigen HBsAg of hepatitis B virus. Transgenic lettuce seedlings were obtained through the application of a regulated balance of plant growth regulators. Genetic transformation process was acquired by cocultivation of cotyledons with Agrobacterium tumefaciens harboring the recombinant plasmid. It is the first description of a lettuce Brazilian variety 'Vitória de Verão' genetically modified.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Research into Future Control of Hepatitis B Virus and Study of Anti-HBC as the Most Sensitive Test for B Virus Carriers in Iran

Two antigen-antibody systems were Out of 42 sera from patients with acute viral hepatitis 33 were positive for HBsAg,all HBsAg positive individuals possessed anti-HBc. From 11 blood donors  sera 8 were positive for HBsAg and 9 were Positive for anti-HBc;2 leprosy sera were tested,both of them were positive for HBsAg and anti-HBc. 5 miscellaneous sam­ples,z·were positive for HBsAg and anti-HBc...

متن کامل

Luteolin-7-O-Glucoside Present in Lettuce Extracts Inhibits Hepatitis B Surface Antigen Production and Viral Replication by Human Hepatoma Cells in Vitro

Hepatitis B virus (HBV) infection is endemic in Asia and chronic hepatitis B (CHB) is a major public health issue worldwide. Current treatment strategies for CHB are not satisfactory as they induce a low rate of hepatitis B surface antigen (HBsAg) loss. Extracts were prepared from lettuce hydroponically cultivated in solutions containing glycine or nitrate as nitrogen sources. The lettuce extra...

متن کامل

Priming Hepatitis B Surface (HBsAg)- and Core Antigen (HBcAg)-Specific Immune Responses by Chimeric, HBcAg with a HBsAg ‘a’ Determinant

We developed an immunogen to stimulate multivalent immunity against hepatitis B surface antigen (HBsAg) and hepatitis B core antigens (HBcAg). Immune responses specific for both HBsAg and HBcAg play an important role in controlling the infection. HBsAg-specific antibodies mediate elimination of virions at an early stage of infection and prevent the spread of virus. The immunogen was constructed...

متن کامل

The Prevalence of Hepatitis B Surface Antigen (HBsAg) and its Influencing Factors in Pregnant Women Referring to Healthcare Centers of Dehloran, Iran in 2011-2012

Background & aim: Hepatitis B virus (HBV) infection is a major global health concern. According to the statistics, the prevalence of this infection is moderate in Iran. Pregnant mothers, who are infected with the virus (virus carriers), can transmit the infection to their fetus. This study aimed to determine the prevalence of hepatitis B surface antigen (HBsAg) and its influencing factors in pr...

متن کامل

Honey Bee Dry Venom Reduces Hepatitis B Virus Surface Antigen Secretion in PLC/PRF/5 Cell Line

Background and Aims: Currently, many efforts are directed toward functional Hepatitis B virus (HBV) treatment. This is achievable by suppression of HBV surface antigen (HBsAg) secretion. In this regard, use of natural products has been the areas of interest by scientific communities. Materials and Methods: Dried Honey Bee venom was extracted for assessing its anti-HBsAg secretion potential. He...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases

دوره 12 6  شماره 

صفحات  -

تاریخ انتشار 2008